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SEC functions also in a very preparative way by trapping the lesser molecules during the pores of the particles. The larger molecules just go through the pores as These are as well big to enter the pores. More substantial molecules therefore stream with the column faster than lesser molecules: which is, the scaled-down the molecule, the lengthier t
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The mixture moves through the column at different velocities and interacts Using the sorbent, often called the stationary phase. The velocity of each and every component while in the combination depends upon 1) its chemical character, two) the character of the column and three) the composition of the mobile stage.Following focus, the sample really
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